Seeking talented molecular biologists to work in an interdisciplinary team environment on understanding how gene regulation is altered in cancer. This position will involve developing, optimizing and validating genome editing technologies. These methods will include CRISPR-Cas9 technologies for geneome editing and genetic perturbation as well as lentivirus production and transduction for the generation of engineered mammalian cell lines. Candidates will be expected to drive technology development and optimization with minimal supervision and to be able to troubleshoot as needed.
- Design experimental approaches with an understanding of how gene regulation is disordered in cancer, how it can be targeted, and the consequences of target pertubation on cell state
- Develop, optimize and run experimental protocols for CRISPR/Cas9 mediated endogenous tagging and/or knockout genes
- Generate CRISPR/lentiviral constructs via molecular cloning techniques including standard restriction, Gateway and Gibson technologies
- Annotate and maintain collaborative protocol repositories for all lab members
- Clearly communicate (oral and written) experimental results and progress
- Work collaboratively with chemical and molecular biologists to engineer mammalian cell lines than can be used to study the mechanism of gene regulation in cancer
Required: Masters degree in a basic science or equivalent. A Bachelor's degree in a basic science and two years of directly related experience will also be considered.
Required: One year of related experience. Competency in one or more key areas including: molecular biology, cell line engineering and gene regulation