A full-time Postdoctoral Associate position is available immediately in the Mitsiades lab at Baylor College of Medicine. We are seeking a highly motivated and skilled scientist to study the SPOP-Cullin3-RBX1 E3 ubiquitin ligase system, including identifying novel substrates of SPOP and elucidating their functional relevance in the context of various cancers. The preferred candidate will be expected to have strong conceptual and experimental background in biochemistry as well as molecular biology, specifically in mammalian cells. Candidates must have at least one first-author publication (or article in press) in a high- impact journal and must demonstrate potential and commitment to become self-funded investigators under proper guidance. Candidates must be enthusiastic, self-motivated, detail-oriented, dedicated to science and problem solving, with organizational skills and effective at working independently and leading projects.
- Design experimental plans, lead study execution, analyze experimental data, generate laboratory protocols, and prepare manuscripts with minimum supervision from senior team leads.
- Troubleshoot and solve difficult problems in the design/execution and development of projects.
- Perform cell/tissue culture. Observe and identify cell changes under the microscope, determine cell counts using the hemocytometer or coulter counter, perform colony counts and determine cell viability.
- Isolate, purify and analyze RNA, DNA and protein including hybridization, gel electrophoresis, ECL imaging, PCR reactions, quantitative RT-PCR assays, etc.
- Perform molecular cloning, transformation, site-specific mutagenesis and transfection of plasmids.
- Perform stable and transient knockdown of targets of interest via siRNA/shRNA, CRISPR, lentiviral constructs, etc.
- Maintain accurate, detailed records of experimental data and protocols.
- Communicate results to and understand instructions from supervisor and other co-workers.
- Fluent spoken English is essential.
- Candidate will be expected to apply for extramural funding.